General Cell Based Assays
Revvity Health Sciences Inc HTRF Human Total SYK Detection Kit, 500 Assay Points
HTRF Human Total SYK Detection Kit, 500 Assay Points
Abcam Cell Cycle Assay Kit (Fluorometric - Green)
Cell Cycle Assay Kit (Fluorometric - Green)
The product is subject to the following: Abcam Restricted Use Statement
ABclonal Technology 12 Lipoxygenase/ALOX12
This gene encodes a member of the lipoxygenase family of proteins The encoded enzyme acts on different polyunsaturated fatty acid substrates to generate bioactive lipid mediators including eicosanoids and lipoxins The encoded enzyme and its reaction products have been shown to regulate platelet function Elevated expression of this gene has been observed in pancreatic islets derived from human diabetes patients Allelic variants in this gene may be associated with susceptibility to toxoplasmosis Multiple pseudogenes of this gene have been identified in the human genome
Eurofins Discoverx cAMP Hunter eXpress GPR35 CHO-K1 GPCR Assay, 2-Plate Kit. (95-0152E2CP2M)
The cAMP Hunter GPR35 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. zaprinast) based activation of GPR35 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GPR35, a naturally Gi/Go coupled GPCR. Upon activation of GPR35, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Abcam Hydroxyproline Assay Kit (Colorimetric)
Hydroxyproline Assay Kit (Colorimetric)
The product is subject to the following: Abcam Restricted Use Statement
Dojindo Molecular Technologies Inc NAD/NADH Assay Kit-WST (100TESTS), enables quantification of the amount of total NAD+/NADH, NAD+ and NADH in cells and measurement of their ratio.
Recently, it has become clear that Sirtuin is linked to longevity and plays a role in NAD+ level regulation. Also Sirtuin has been recognized as a marker necessary to understanding biological states, such as obesity & diabetes, as well as cellular differentiation. Cell lysate from a cell culture can be easily prepared via Deproteinzation using the extraction buffer and filtration tubes found within this kit. Intracellular NADH levels can be quantified by heat treatment of cell lysate. Additionally, intracellular NAD+ levels can be determined by subtracting NADH levels from independently measured total NAD+/NADH levels. NAD/NADH Assay Kit-WST enables quantification of the amount of total NAD+/NADH, NADH and NAD+ in cells and measurement of their ratio. Dojindo has various quantification kits of Intracellular Metabolites. (https://www.dojindo.com/quantification-of-intracellular-metabolites/)
Eurofins Discoverx PathHunter eXpress mOPRK1 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0480E2MCP0M)
The PathHunter mOPRK1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. dynorphin A) based activation of mOPRK1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mOPRK1 and EA-tagged β-Arrestin-2. Activation of mOPRK1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cayman Chemical SodIM HydroxID asy Reagen 10mL
An LHR antagonist (IC50s 185 and 46 nM for the human and rat receptors respectively) selective for LHR over TSH and FSH receptors (IC50s 24 and >16 UM respectively for the human receptors) and a panel of 25 GPCRs at 10 UM reduces serum levels of estradiol in female rats at 12.5 mg/kg per day
GPCR Kinase 5 Expression Kit - BacMam, 10mL - Montana Molecular
GPCR Kinase 5 (GRK5) packaged in BacMam for expression in mammalian cells. Expression is easily titrateable to your experimental needs or to adjust assay sensitivity, enabling rapid assay optimization. Co-express with other Montana Molecular tools includeing GPCRs packaged in BacMam or biosensors for cAMP, DAG, or arrestin signaling.